NPTFit is a specialized Python/Cython package that implements Non-Poissonian Template Fitting (NPTF), originally developed for characterizing populations of unresolved point sources. It offers fast evaluation of likelihoods for NPTF analyses and has an easy-to-use interface for performing non-Poissonian (as well as standard Poissonian) template fits using MultiNest (ascl:1109.006) or other inference tools. It allows inclusion of an arbitrary number of point source templates, with an arbitrary number of degrees of freedom in the modeled flux distribution, and has modules for analyzing and plotting the results of an NPTF.