PyNeb (previously PyNebular) is an update and expansion of the IRAF package NEBULAR; rewritten in Python, it is designed to be more user-friendly and powerful, increasing the speed, easiness of use, and graphic visualization of emission lines analysis. In PyNeb, the atom is represented as an n-level atom. For given density and temperature, PyNeb solves the equilibrium equations and determines the level populations. PyNeb can compute physical conditions from suitable diagnostic line ratios and level populations, critical densities and line emissivities, and can compute and display emissivity grids as a function of Te and Ne. It can also deredden line intensities, read and manage observational data, and plot and compare atomic data from different publications, and compute ionic abundances from line intensities and physical conditions and elemental abundances from ionic abundances and icfs.