pyHIIexplorerV2 extracts the integrated spectra of HII regions from integral field spectroscopy (IFS) datacubes. The detection of HII regions performed by pyHIIexplorer is based on two assumptions: 1) HII regions have strong emission lines that are clearly above the continuum emission and the average ionized gas emission across each galaxy, and 2) the typical size of HII regions is about a few hundreds of parsecs, which corresponds to a usual projected size of a few arcsec at the distance of our galaxies. These assumptions will define clumpy structures with a high Ha emission line contrast in comparison to the continuum. pyHIIexplorerV2 is written in Python; it is based on and is a successor to HIIexplorer (ascl:1603.017).